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PalDat - Palynological Database

an online publication on recent pollen

Helleborus foetidus

Taxonomy: Angiospermae, Ranunculales, Ranunculaceae, Helleborus

Links: http://flora.nhm-wien.ac.at/seiten-arten/helleborus-foetidus.htm

Published: 2021-06-23

Pollen Description

Shape, Size and Aperture

pollen unit: monad,  dispersal unit and peculiarities: monad,  size (pollen unit): small (10-25 µm),  size of hydrated pollen (LM): 21-25 µm, 26-30 µm,  shortest polar axis in equatorial view (LM): 21-25 µm,  longest polar axis in equatorial view (LM): -,  shortest diameter in equatorial or polar view (LM): 26-30 µm,  longest diameter in equatorial or polar view (LM): 26-30 µm,  pollen class: colpate,  polarity: isopolar,  P/E-ratio: oblate,  shape: -,  outline in polar view: circular,  dominant orientation (LM): oblique,  P/E-ratio (dry pollen): prolate,  shape (dry pollen): -,  outline in polar view (dry pollen): lobate,  infoldings (dry pollen): aperture(s) sunken,  aperture number: 3,  aperture type: colpus,  aperture condition: colpate, tricolpate,  aperture peculiarities: aperture membrane ornamented

Ornamentation and Structure

LMornamentation LM: scabrate, verrucate, gemmate,  nexine: -,  sexine: -,  SEMornamentation SEM: microreticulate, microverrucate,  suprasculpture SEM: -,  TEMtectum: semitectate,  infratectum: columellate,  foot layer: continuous,  endexine: compact-continuous,  intine: monolayered,  wall peculiarities: -,  supratectal element: -

Miscellaneous

pollen coatings: pollenkitt,  reserves in cytoplasm: starch, lipids,  cell number: 2-celled,  Ubisch bodies: present

Author(s) of diagnosis: Auer, Angelika; Halbritter, Heidemarie; Auer, Waltraud

Pictures

flower(s)

1. flower(s)

polar view (proximal)

2. polar view (proximal)

optical section

3. optical section

polar view (distal)

4. polar view (distal)

generative cell (asterisk) and vegetative nucleus

5. generative cell (asterisk) and vegetative nucleus

hydrated Pollen

6. hydrated Pollen

hydrated Pollen

7. hydrated Pollen

hydrated Pollen

8. hydrated Pollen

hydrated pollen grains

9. hydrated pollen grains

polar view

10. polar view

equatorial view

11. equatorial view

aperture

12. aperture

detail of aperture

13. detail of aperture

exine surface

14. exine surface

polar area

15. polar area

dry pollen grains

16. dry pollen grains

Ubisch bodies in loculus

17. Ubisch bodies in loculus

interapertural area of pollen wall, intine (I), endexine (E)

18. interapertural area of pollen wall, intine (I), endexine (E)

apertural area of pollen wall, intine (I), endexine (E)

19. apertural area of pollen wall, intine (I), endexine (E)

tapetum cells with Ubisch bodies (U)

20. tapetum cells with Ubisch bodies (U)

Picture legend

  1. flower(s), photographer: Auer, A.
  2. polar view (proximal) - fresh, acetolyzed, unstained, photographer: Auer, A.
  3. optical section - fresh, acetolyzed, unstained, photographer: Auer, A.
  4. polar view (distal) - fresh, acetolyzed, unstained, photographer: Auer, A.
  5. generative cell (asterisk) and vegetative nucleus - fixed, formalin-acetic-alcohol, aceto-carmine, photographer: Auer, A.
  6. hydrated Pollen - fresh, glycerine, unstained, photographer: Auer, W.
  7. hydrated Pollen - fresh, glycerine, ruthenium red, photographer: Auer, W.
  8. hydrated Pollen - fresh, glycerine, ruthenium red, photographer: Auer, W.
  9. hydrated pollen grains - fresh, rehydrated (water) & critical point dried & sputter coated with gold, photographer: Halbritter, H.
  10. polar view - fresh, rehydrated (water) & critical point dried & sputter coated with gold, photographer: Halbritter, H.
  11. equatorial view - fresh, rehydrated (water) & critical point dried & sputter coated with gold, photographer: Halbritter, H.
  12. aperture - fresh, rehydrated (water) & critical point dried & sputter coated with gold, photographer: Halbritter, H.
  13. detail of aperture - fresh, rehydrated (water) & critical point dried & sputter coated with gold, photographer: Halbritter, H.
  14. exine surface - fresh, rehydrated (water) & critical point dried & sputter coated with gold, photographer: Halbritter, H.
  15. polar area - fresh, rehydrated (water) & critical point dried & sputter coated with gold, photographer: Halbritter, H.
  16. dry pollen grains - fresh, sputter coated with gold, photographer: Halbritter, H.
  17. Ubisch bodies in loculus - fresh, rehydrated (water) & critical point dried & sputter coated with gold, photographer: Halbritter, H.
  18. interapertural area of pollen wall, intine (I), endexine (E) - fresh, glutaraldehyde & osmium & potassium ferrocyanide, modified Thiéry-test, photographer: Auer, A.
  19. apertural area of pollen wall, intine (I), endexine (E) - fresh, glutaraldehyde & osmium & potassium ferrocyanide, modified Thiéry-test, photographer: Auer, A.
  20. tapetum cells with Ubisch bodies (U) - fresh, glutaraldehyde & osmium & potassium ferrocyanide, uranyl acetate & lead citrate, photographer: Auer, A.

Literature

  • Echlin P, Godwin H (1968) The ultrastructure and ontogeny of pollen in Helleborus foetidus L. I. The development of the tapetum and ubisch bodies. J Cell Sci 3: 161-174
  • Echlin P, Godwin H (1968) The ultrastructure and ontogeny of pollen in Helleborus foetidus L. II. Pollen grain development through the callose special wall stage. J Cell Sci 3: 175-186
  • Echlin P, Godwin H (1969) The ultrastructure and ontogeny of pollen in Helleborus foetidus L. III. The formation of the pollen grain wall. J Cell Sci 5: 459-477
  • Heslop-Harrison J, Heslop-Harrison JS, Heslop-Harrison Y (1986) The comportment of the vegetative nucleus and generative cell in the pollen and pollen tubes of Helleborus foetidus L. Ann Bot 58: 1-12
  • Nowicke JW, Skvarla JJ (1983) A palynological study of the genus Helleborus (Ranunculaceae). Grana 22: 129-140
  • Oberschneider W (1998) Pollenmorphologie der Ranunculanae. Diplomarbeit. Universität Wien : 125 pp
  • Adler W, Oswald K, Fischer R. (1994) Exkursionsflora von Österreich : 1180
  • Halbritter, H (1998) Preparing living pollen material for scanning electron microscopy using 2,2-dimethoxypropane (DMP) and criticalpoint drying. Biotechnic Histochem 73: 137–143

Copyright and Citation

Cite this publication as:

Auer A., Halbritter H., Auer W. 2021. Helleborus foetidus. In: PalDat - A palynological database. https://www.paldat.org/pub/Helleborus_foetidus/306247;jsessionid=3751B29D255D31D56ACDA11A5510E9BD; accessed 2021-07-27

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