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PalDat - Palynological Database

an online publication on recent pollen

Helleborus niger

Taxonomy: Angiospermae, Ranunculales, Ranunculaceae, Helleborus

Published: 2016-10-21

Pollen Description

Shape, Size and Aperture

pollen unit: monad,  dispersal unit and peculiarities: monad,  size (pollen unit): medium-sized (26-50 µm),  pollen class: colpate,  polarity: isopolar,  shape: oblate,  outline in polar view: circular,  shape (dry pollen): prolate,  outline in polar view (dry pollen): lobate,  infoldings (dry pollen): aperture(s) sunken,  aperture number: 3,  aperture type: colpus,  aperture condition: colpate, tricolpate,  aperture peculiarities: aperture membrane ornamented

Ornamentation and Structure

LMornamentation LM: scabrate,  nexine: -,  sexine: -,  SEMornamentation SEM: perforate, microechinate,  TEMtectum: eutectate,  infratectum: columellate,  foot layer: continuous,  endexine: compact-continuous,  intine: monolayered,  wall peculiarities: -

Miscellaneous

pollen coatings: pollenkitt,  reserves in cytoplasm: starch, lipids,  cell number: 2-celled,  Ubisch bodies: present

Annotations

shape oblate to spheroidal

Author(s) of diagnosis: Oberschneider, Wolfgang; Halbritter, Heidemarie

Pictures

flower(s)

1. flower(s)

upper focus

2. upper focus

optical section

3. optical section

lower focus

4. lower focus

generative cell (left) and vegetative nucleus

5. generative cell (left) and vegetative nucleus

hydrated pollen grains

6. hydrated pollen grains

polar view (oblique)

7. polar view (oblique)

equatorial view

8. equatorial view

aperture

9. aperture

detail of aperture

10. detail of aperture

exine surface

11. exine surface

polar area

12. polar area

dry pollen grains

13. dry pollen grains

Ubisch bodies in loculus

14. Ubisch bodies in loculus

interapertural area of pollen wall

15. interapertural area of pollen wall

aperture

16. aperture

pollen wall and tapetum cells with Ubisch bodies (asterisks)

17. pollen wall and tapetum cells with Ubisch bodies (asterisks)

Picture legend

  1. flower(s), photographer: Oberschneider, W.
  2. upper focus - fresh, acetolyzed, unstained, photographer: Oberschneider, W.
  3. optical section - fresh, acetolyzed, unstained, photographer: Oberschneider, W.
  4. lower focus - fresh, acetolyzed, unstained, photographer: Oberschneider, W.
  5. generative cell (left) and vegetative nucleus - fixed, formalin-acetic-alcohol, aceto-carmine, photographer: Oberschneider, W.
  6. hydrated pollen grains - fresh, rehydrated (water) & critical point dried & sputter coated with gold, photographer: Halbritter, H.
  7. polar view (oblique) - fresh, rehydrated (water) & critical point dried & sputter coated with gold, photographer: Halbritter, H.
  8. equatorial view - fresh, rehydrated (water) & critical point dried & sputter coated with gold, photographer: Halbritter, H.
  9. aperture - fresh, rehydrated (water) & critical point dried & sputter coated with gold, photographer: Halbritter, H.
  10. detail of aperture - fresh, rehydrated (water) & critical point dried & sputter coated with gold, photographer: Halbritter, H.
  11. exine surface - fresh, rehydrated (water) & critical point dried & sputter coated with gold, photographer: Halbritter, H.
  12. polar area - fresh, rehydrated (water) & critical point dried & sputter coated with gold, photographer: Halbritter, H.
  13. dry pollen grains - dry, sputter coated with gold, photographer: Halbritter, H.
  14. Ubisch bodies in loculus - fresh, rehydrated (water) & critical point dried & sputter coated with gold, photographer: Halbritter, H.
  15. interapertural area of pollen wall - fresh, glutaraldehyde & osmium & potassium ferrocyanide, uranyl acetate & lead citrate, photographer: Oberschneider, W.
  16. aperture - fresh, glutaraldehyde & osmium & potassium ferrocyanide, uranyl acetate & lead citrate, photographer: Oberschneider, W.
  17. pollen wall and tapetum cells with Ubisch bodies (asterisks) - fresh, glutaraldehyde & osmium & potassium ferrocyanide, uranyl acetate & lead citrate, photographer: Oberschneider, W.

Literature

  • Echlin P, Godwin H (1968) The ultrastructure and ontogeny of pollen in Helleborus foetidus L. I. The development of the tapetum and ubisch bodies. J Cell Sci 3: 161-174
  • Echlin P, Godwin H (1968) The ultrastructure and ontogeny of pollen in Helleborus foetidus L. II. Pollen grain development through the callose special wall stage. J Cell Sci 3: 175-186
  • Echlin P, Godwin H (1969) The ultrastructure and ontogeny of pollen in Helleborus foetidus L. III. The formation of the pollen grain wall. J Cell Sci 5: 459-477
  • Heslop-Harrison J, Heslop-Harrison JS, Heslop-Harrison Y (1986) The comportment of the vegetative nucleus and generative cell in the pollen and pollen tubes of Helleborus foetidus L. Ann Bot 58: 1-12
  • Nowicke JW, Skvarla JJ (1983) A palynological study of the genus Helleborus (Ranunculaceae). Grana 22: 129-140
  • Oberschneider W (1998) Pollenmorphologie der Ranunculanae. Diplomarbeit. Universität Wien : 125 pp
  • Adler W, Oswald K, Fischer R. (1994) Exkursionsflora von Österreich : 1180
  • Halbritter, H (1998) Preparing living pollen material for scanning electron microscopy using 2,2-dimethoxypropane (DMP) and criticalpoint drying. Biotechnic Histochem 73: 137–143

Copyright and Citation

Cite this publication as:

Oberschneider W., Halbritter H. 2016. Helleborus niger. In: PalDat - A palynological database. https://www.paldat.org/pub/Helleborus_niger/301722;jsessionid=3E6E52C77F83C8731E6BE958170C4551; accessed 2018-11-18

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