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an online publication on recent pollen

Populus nigra

Taxonomy: Angiospermae, Malpighiales, Salicaceae, Populus

Published: 2020-07-29

Pollen Description

Shape, Size and Aperture

pollen unit: monad,  dispersal unit and peculiarities: monad,  size (pollen unit): medium-sized (26-50 µm),  size of hydrated pollen (LM): 26-30 µm,  shortest polar axis in equatorial view (LM): -,  longest polar axis in equatorial view (LM): -,  shortest diameter in equatorial or polar view (LM): 26-30 µm,  longest diameter in equatorial or polar view (LM): 26-30 µm,  pollen class: inaperturate,  polarity: isopolar,  P/E-ratio: -,  shape: spheroidal,  outline in polar view: circular,  dominant orientation (LM): oblique,  P/E-ratio (dry pollen): -,  shape (dry pollen): irregular,  outline in polar view (dry pollen): irregular,  infoldings (dry pollen): irregularly infolded,  aperture number: none,  aperture type: no aperture,  aperture condition: inaperturate,  aperture peculiarities: -

Ornamentation and Structure

LMornamentation LM: scabrate, verrucate, gemmate,  nexine: -,  sexine: -,  SEMornamentation SEM: no suitable term,  suprasculpture SEM: -,  TEMtectum: semitectate,  infratectum: columellate,  foot layer: discontinuous,  endexine: compact-continuous,  intine: monolayered,  wall peculiarities: -,  supratectal element: -

Miscellaneous

pollen coatings: absent,  reserves in cytoplasm: starch,  cell number: 2-celled,  Ubisch bodies: present

Annotations: endexine thin; ornamentation occasional microechinate

Author(s) of diagnosis: Halbritter, Halbritter; Sam, Saskia; Heigl, Helmut

Pictures

habitus

1. habitus

inflorescence(s)

2. inflorescence(s)

acetolyzed pollen,upper focus

3. acetolyzed pollen, upper focus

acetolyzed pollen,optical section

4. acetolyzed pollen, optical section

acetolyzed pollen,lower focus

5. acetolyzed pollen, lower focus

generative cell

6. generative cell

hydrated Pollen

7. hydrated Pollen

hydrated Pollen

8. hydrated Pollen

hydrated Pollen

9. hydrated Pollen

hydrated pollen grains

10. hydrated pollen grains

hydrated pollen grain

11. hydrated pollen grain

exine surface

12. exine surface

exine surface

13. exine surface

dry pollen grains

14. dry pollen grains

Ubisch bodies on locule wall

15. Ubisch bodies on locule wall

pollen grain in cross section

16. pollen grain in cross section

pollen wall, interapertural area

17. pollen wall, interapertural area

pollen wall, interapertural area

18. pollen wall, interapertural area

vegetative nucleus and generative cell

19. vegetative nucleus and generative cell

Ubisch bodies on locule wall

20. Ubisch bodies on locule wall

Picture legend

  1. habitus, photographer: Sam, S.
  2. inflorescence(s), photographer: Sam, S.
  3. acetolyzed pollen, upper focus - dry, acetolyzed, unstained, photographer: Sam, S.
  4. acetolyzed pollen, optical section - dry, acetolyzed, unstained, photographer: Sam, S.
  5. acetolyzed pollen, lower focus - dry, acetolyzed, unstained, photographer: Sam, S.
  6. generative cell - fresh, unfixed, aceto-carmine, photographer: Sam, S.
  7. hydrated Pollen - fresh, glycerine, unstained, photographer: Heigl, H.
  8. hydrated Pollen - fresh, glycerine, ruthenium red, photographer: Heigl, H.
  9. hydrated Pollen - fresh, glycerine, ruthenium red, photographer: Heigl, H.
  10. hydrated pollen grains - fresh, DMP & critical point dried, photographer: Halbritter, H.
  11. hydrated pollen grain - fresh, DMP & critical point dried, photographer: Halbritter, H.
  12. exine surface - fresh, DMP & critical point dried, photographer: Halbritter, H.
  13. exine surface - fresh, rehydrated (water) & critical point dried & sputter coated with gold, photographer: Sam, S.
  14. dry pollen grains - dry, sputter coated with gold, photographer: Halbritter, H.
  15. Ubisch bodies on locule wall - fresh, rehydrated (water) & critical point dried & sputter coated with gold, photographer: Sam, S.
  16. pollen grain in cross section - fresh, glutaraldehyde & osmium & potassium ferrocyanide, modified Thiéry-test, photographer: Sam, S.
  17. pollen wall, interapertural area - fresh, glutaraldehyde & osmium & potassium ferrocyanide, modified Thiéry-test, photographer: Sam, S.
  18. pollen wall, interapertural area - fresh, glutaraldehyde & osmium & potassium ferrocyanide, potassium permanganate, photographer: Sam, S.
  19. vegetative nucleus and generative cell - fresh, glutaraldehyde & osmium & potassium ferrocyanide, modified Thiéry-test, photographer: Sam, S.
  20. Ubisch bodies on locule wall - fresh, glutaraldehyde & osmium & potassium ferrocyanide, modified Thiéry-test, photographer: Sam, S.

Literature

  • Adler W, Oswald K, Fischer R. (1994) Exkursionsflora von Österreich : 1180
  • Halbritter, H (1998) Preparing living pollen material for scanning electron microscopy using 2,2-dimethoxypropane (DMP) and criticalpoint drying. Biotechnic Histochem 73: 137–143

Copyright and Citation

Cite this publication as:

Halbritter H., Sam S., Heigl H. 2020. Populus nigra. In: PalDat - A palynological database. https://www.paldat.org/pub/Populus_nigra/303756;jsessionid=7B2A095883912D954D00DAD2600123FE; accessed 2020-09-19

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