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PalDat - Palynological Database

an online publication on recent pollen

Pulsatilla grandis

Taxonomy: Angiospermae, Ranunculales, Ranunculaceae, Pulsatilla

Links: http://flora.nhm-wien.ac.at/seiten-arten/pulsatilla-grandis.htm

Published: 2021-01-27

Pollen Description

Shape, Size and Aperture

pollen unit: monad,  dispersal unit and peculiarities: monad,  size (pollen unit): medium-sized (26-50 µm),  size of hydrated pollen (LM): 36-40 µm, 41-50 µm,  shortest polar axis in equatorial view (LM): 31-35 µm,  longest polar axis in equatorial view (LM): 41-50 µm,  shortest diameter in equatorial or polar view (LM): 36-40 µm,  longest diameter in equatorial or polar view (LM): 36-40 µm,  pollen class: colpate,  polarity: isopolar,  P/E-ratio: -,  shape: spheroidal,  outline in polar view: circular,  dominant orientation (LM): oblique,  P/E-ratio (dry pollen): prolate,  shape (dry pollen): -,  outline in polar view (dry pollen): lobate,  infoldings (dry pollen): aperture(s) sunken,  aperture number: 3,  aperture type: colpus,  aperture condition: colpate, tricolpate,  aperture peculiarities: aperture membrane ornamented

Ornamentation and Structure

LMornamentation LM: scabrate, verrucate, gemmate,  nexine: -,  sexine: -,  SEMornamentation SEM: microechinate, perforate,  suprasculpture SEM: -,  TEMtectum: eutectate,  infratectum: columellate,  foot layer: continuous,  endexine: compact-continuous,  intine: monolayered,  wall peculiarities: -,  supratectal element: -

Miscellaneous

pollen coatings: pollenkitt,  reserves in cytoplasm: starch, lipids,  cell number: 2-celled,  Ubisch bodies: present

Author(s) of diagnosis: Oberschneider, Wolfgang; Auer, Waltraud

Pictures

flower(s)

1. flower(s)

flower(s)

2. flower(s)

upper focus

3. upper focus

optical section

4. optical section

lower focus

5. lower focus

generative cell (dark) and vegetative nucleus

6. generative cell (dark) and vegetative nucleus

hydrated pollen

7. hydrated pollen

hydrated pollen

8. hydrated pollen

hydrated pollen

9. hydrated pollen

polar view

10. polar view

polar view

11. polar view

equatorial view

12. equatorial view

detail of aperture

13. detail of aperture

exine surface

14. exine surface

polar view of dry pollen grain

15. polar view of dry pollen grain

dry pollen grain in equatorial view

16. dry pollen grain in equatorial view

interapertural area of pollen wall

17. interapertural area of pollen wall

aperture

18. aperture

pollen wall and tapetum cells with Ubisch bodies (asterisk)

19. pollen wall and tapetum cells with Ubisch bodies (asterisk)

Picture legend

  1. flower(s), photographer: Svojtka, M.
  2. flower(s), photographer: Svojtka, M.
  3. upper focus - fresh, acetolyzed, unstained, photographer: Oberschneider, W.
  4. optical section - fresh, acetolyzed, unstained, photographer: Oberschneider, W.
  5. lower focus - fresh, acetolyzed, unstained, photographer: Oberschneider, W.
  6. generative cell (dark) and vegetative nucleus - fixed, formalin-acetic-alcohol, aceto-carmine, photographer: Oberschneider, W.
  7. hydrated pollen - fresh, glycerine, unstained, photographer: Auer, W.
  8. hydrated pollen - fresh, glycerine, ruthenium red, photographer: Auer, W.
  9. hydrated pollen - fresh, glycerine, ruthenium red, photographer: Auer, W.
  10. polar view - fresh, rehydration (water) & chloroform & critical point dried & sputter coated with gold, photographer: Oberschneider, W.
  11. polar view - fresh, rehydration (water) & chloroform & critical point dried & sputter coated with gold, photographer: Oberschneider, W.
  12. equatorial view - fresh, rehydration (water) & chloroform & critical point dried & sputter coated with gold, photographer: Oberschneider, W.
  13. detail of aperture - fresh, rehydration (water) & chloroform & critical point dried & sputter coated with gold, photographer: Oberschneider, W.
  14. exine surface - fresh, rehydration (water) & chloroform & critical point dried & sputter coated with gold, photographer: Oberschneider, W.
  15. polar view of dry pollen grain - dry, sputter coated with gold, photographer: Oberschneider, W.
  16. dry pollen grain in equatorial view - dry, sputter coated with gold, photographer: Oberschneider, W.
  17. interapertural area of pollen wall - fresh, glutaraldehyde & osmium & potassium ferrocyanide, modified Thiéry-test, photographer: Oberschneider, W.
  18. aperture - fresh, glutaraldehyde & osmium & potassium ferrocyanide, uranyl acetate & lead citrate, photographer: Oberschneider, W.
  19. pollen wall and tapetum cells with Ubisch bodies (asterisk) - fresh, glutaraldehyde & osmium & potassium ferrocyanide, modified Thiéry-test, photographer: Oberschneider, W.

Literature

  • Halbritter, H (1998) Preparing living pollen material for scanning electron microscopy using 2,2-dimethoxypropane (DMP) and criticalpoint drying. Biotechnic Histochem 73: 137–143
  • Adler W, Oswald K, Fischer R. (1994) Exkursionsflora von Österreich : 1180

Copyright and Citation

Cite this publication as:

Oberschneider W., Auer W., Svojtka M. 2021. Pulsatilla grandis. In: PalDat - A palynological database. https://www.paldat.org/pub/Pulsatilla_grandis/305136;jsessionid=385E2A747BFDF01F2A7E1EDED6433442; accessed 2021-07-27

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